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ZÍTKA, O. KŘÍŽKOVÁ, S. SKALIČKOVÁ, S. FIALOVÁ, D. ADAM, V. KIZEK, R.
Originální název
Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Lactoferrin (LF) is approximately 80 kDa iron-binding protein, which is important part of saliva and other body fluids. Due to its ability to bind metal ions, it has many biologically important functions. In this study, a method for the isolation of LF from a biological sample using robotically prepared antibody-modified paramagnetic particles was developed using robotic pipetting station. The method consisted of the following optimised steps. Protein G was bound on the paramagnetic particles, on which goat antibody (10 microg) was linked. LF was subsequently added to microtitration plate, which had affinity to goat antibody and the interaction lasted for 30 min. We found that the highest signals were obtained using the combination of goat antibody 1:3000, murine antibody 1:5000 and conjugate 1:1500
Klíčová slova
Microfluidic tool, elektrochemical detection, lactoferrin
Autoři
ZÍTKA, O.; KŘÍŽKOVÁ, S.; SKALIČKOVÁ, S.; FIALOVÁ, D.; ADAM, V.; KIZEK, R.
Rok RIV
2013
Vydáno
1. 7. 2013
ISSN
0173-0835
Periodikum
Electrophoresis
Ročník
34
Číslo
14
Stát
Spolková republika Německo
Strany od
2120
Strany do
2128
Strany počet
9
BibTex
@article{BUT103092, author="Ondřej {Zítka} and Soňa {Křížková} and Sylvie {Skaličková} and Dana {Fialová} and Vojtěch {Adam} and René {Kizek}", title="Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads", journal="Electrophoresis", year="2013", volume="34", number="14", pages="2120--2128", doi="10.1002/elps.201200631", issn="0173-0835" }