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KREJČOVÁ, L. NEJDL, L. HYNEK, D. KŘÍŽKOVÁ, S. KOPEL, P. ADAM, V. KIZEK, R.
Original Title
Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots
Type
journal article in Web of Science
Language
English
Original Abstract
In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.
Keywords
influenza hemagglutinin; electrochemical detection; paramagnetic particles; glycan; quantum dot labeling
Authors
KREJČOVÁ, L.; NEJDL, L.; HYNEK, D.; KŘÍŽKOVÁ, S.; KOPEL, P.; ADAM, V.; KIZEK, R.
RIV year
2013
Released
13. 12. 2013
Publisher
MDPI
ISBN
1420-3049
Periodical
MOLECULES
Year of study
18
Number
12
State
Swiss Confederation
Pages from
15573
Pages to
15586
Pages count
14
URL
https://www.mdpi.com/1420-3049/18/12/15573
Full text in the Digital Library
http://hdl.handle.net/11012/180499
BibTex
@article{BUT103909, author="Ludmila {Krejčová} and Lukáš {Nejdl} and David {Hynek} and Soňa {Křížková} and Pavel {Kopel} and Vojtěch {Adam} and René {Kizek}", title="Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots", journal="MOLECULES", year="2013", volume="18", number="12", pages="15573--15586", doi="10.3390/molecules181215573", issn="1420-3049", url="https://www.mdpi.com/1420-3049/18/12/15573" }