Detail publikace

Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

KREJČOVÁ, L. NEJDL, L. HYNEK, D. KŘÍŽKOVÁ, S. KOPEL, P. ADAM, V. KIZEK, R.

Originální název

Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.

Klíčová slova

influenza hemagglutinin; electrochemical detection; paramagnetic particles; glycan; quantum dot labeling

Autoři

KREJČOVÁ, L.; NEJDL, L.; HYNEK, D.; KŘÍŽKOVÁ, S.; KOPEL, P.; ADAM, V.; KIZEK, R.

Rok RIV

2013

Vydáno

13. 12. 2013

Nakladatel

MDPI

ISSN

1420-3049

Periodikum

MOLECULES

Ročník

18

Číslo

12

Stát

Švýcarská konfederace

Strany od

15573

Strany do

15586

Strany počet

14

URL

https://www.mdpi.com/1420-3049/18/12/15573

Plný text v Digitální knihovně

http://hdl.handle.net/11012/180499

BibTex

@article{BUT103909,
  author="Ludmila {Krejčová} and Lukáš {Nejdl} and David {Hynek} and Soňa {Křížková} and Pavel {Kopel} and Vojtěch {Adam} and René {Kizek}",
  title="Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots",
  journal="MOLECULES",
  year="2013",
  volume="18",
  number="12",
  pages="15573--15586",
  doi="10.3390/molecules181215573",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/18/12/15573"
}