Publication detail

Utilization of waste material for immobilization of cellulose-degrading enzymes

ZICHOVÁ, M. STRATILOVÁ, E. OMELKOVÁ, J. ROSENBERG, M.

Original Title

Utilization of waste material for immobilization of cellulose-degrading enzymes

Type

abstract

Language

English

Original Abstract

Great amounts of cellulosic wastes are produced worldwide as residues from agriculture and food industry. Cellulose is a polymer of glucose and it is the most abundant organic material in nature. It is however resistant to decomposition. The degradation of cellulose is done by enzymes and the enzyme system consists of at least three components, an exoglucanase, an endoglucanase and a beta-glucosidase. In addition, reducing sugars as end products of enzymatic hydrolysis can be fermented by yeast or bacteria to other valuable metabolites, e.g. bioethanol, a promising alternative fuel nowadays. The use of microorganisms and enzymes in order to remove or reduce cellulosic wastes is a real environmental challenge, which could be solved by a focused research concerning efficient methods applied in biological degradation processes [1]. The purpose of this work is to present the possibility of the biodegradation of cellulosic wastes by immobilized enzymes. Cellulase enzyme of Trichoderma was immobilized by using crushed polyethylene terephthalate (PET) bottles obtained from wastes as carrier. The properties of the immobilized cellulase were investigated such as the optimum pH, optimum temperature, thermal stability, storage stability and operational stability. The obtained results were compared with free enzyme and two other commercial carriers (Sorsilen, Eupergit C) and they showed that PET carrier is effective; in addition this carrier is environmental-friendly (reuse of waste) and economical alternative for many commercial and expensive carriers available.

Keywords

immobilization, cellulase, carrier

Authors

ZICHOVÁ, M.; STRATILOVÁ, E.; OMELKOVÁ, J.; ROSENBERG, M.

Released

3. 12. 2014

Location

Brno

ISBN

978-80-214-5073-8

Book

EMEC15 - The 15th European Meeting on Environmental Chemistry

BibTex

@misc{BUT110977,
  author="Miroslava {Zichová} and Eva {Stratilová} and Jiřina {Omelková} and Michal {Rosenberg}",
  title="Utilization of waste material for immobilization of cellulose-degrading enzymes",
  booktitle="EMEC15 - The 15th European Meeting on Environmental Chemistry",
  year="2014",
  address="Brno",
  isbn="978-80-214-5073-8",
  note="abstract"
}