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ZICHOVÁ, M. STRATILOVÁ, E. OMELKOVÁ, J. ROSENBERG, M.
Originální název
Utilization of waste material for immobilization of cellulose-degrading enzymes
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Great amounts of cellulosic wastes are produced worldwide as residues from agriculture and food industry. Cellulose is a polymer of glucose and it is the most abundant organic material in nature. It is however resistant to decomposition. The degradation of cellulose is done by enzymes and the enzyme system consists of at least three components, an exoglucanase, an endoglucanase and a beta-glucosidase. In addition, reducing sugars as end products of enzymatic hydrolysis can be fermented by yeast or bacteria to other valuable metabolites, e.g. bioethanol, a promising alternative fuel nowadays. The use of microorganisms and enzymes in order to remove or reduce cellulosic wastes is a real environmental challenge, which could be solved by a focused research concerning efficient methods applied in biological degradation processes [1]. The purpose of this work is to present the possibility of the biodegradation of cellulosic wastes by immobilized enzymes. Cellulase enzyme of Trichoderma was immobilized by using crushed polyethylene terephthalate (PET) bottles obtained from wastes as carrier. The properties of the immobilized cellulase were investigated such as the optimum pH, optimum temperature, thermal stability, storage stability and operational stability. The obtained results were compared with free enzyme and two other commercial carriers (Sorsilen, Eupergit C) and they showed that PET carrier is effective; in addition this carrier is environmental-friendly (reuse of waste) and economical alternative for many commercial and expensive carriers available.
Klíčová slova
immobilization, cellulase, carrier
Autoři
ZICHOVÁ, M.; STRATILOVÁ, E.; OMELKOVÁ, J.; ROSENBERG, M.
Vydáno
3. 12. 2014
Místo
Brno
ISBN
978-80-214-5073-8
Kniha
EMEC15 - The 15th European Meeting on Environmental Chemistry
BibTex
@misc{BUT110977, author="Miroslava {Zichová} and Eva {Stratilová} and Jiřina {Omelková} and Michal {Rosenberg}", title="Utilization of waste material for immobilization of cellulose-degrading enzymes", booktitle="EMEC15 - The 15th European Meeting on Environmental Chemistry", year="2014", address="Brno", isbn="978-80-214-5073-8", note="abstract" }