Publication detail

Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

HUDEČKOVÁ, H. ŠUPINOVÁ, P. BABÁK, L.

Original Title

Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

Type

abstract

Language

English

Original Abstract

The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used α amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for α amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 g∙L-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 g∙L 1.

Keywords

waste bread, enzymatic hydrolysis, amylases, alpha-amylase, glucoamylase, pH, temperature

Authors

HUDEČKOVÁ, H.; ŠUPINOVÁ, P.; BABÁK, L.

RIV year

2014

Released

20. 10. 2014

Location

Ústav analytické chemie AV ČR, v. v. i.

ISBN

978-80-904959-2-0

Book

CECE 2014 11th International Interdisciplinary Meeting on Bioanalysis

Pages from

252

Pages to

255

Pages count

475

URL

BibTex

@misc{BUT111690,
  author="Helena {Hudečková} and Petra {Šupinová} and Libor {Babák}",
  title="Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread",
  booktitle="CECE 2014 11th International Interdisciplinary Meeting on Bioanalysis",
  year="2014",
  pages="252--255",
  address="Ústav analytické chemie AV ČR, v. v. i.",
  isbn="978-80-904959-2-0",
  url="http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf",
  note="abstract"
}