Detail publikace

Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

HUDEČKOVÁ, H. ŠUPINOVÁ, P. BABÁK, L.

Originální název

Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used α amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for α amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 g∙L-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 g∙L 1.

Klíčová slova

waste bread, enzymatic hydrolysis, amylases, alpha-amylase, glucoamylase, pH, temperature

Autoři

HUDEČKOVÁ, H.; ŠUPINOVÁ, P.; BABÁK, L.

Rok RIV

2014

Vydáno

20. 10. 2014

Místo

Ústav analytické chemie AV ČR, v. v. i.

ISBN

978-80-904959-2-0

Kniha

CECE 2014 11th International Interdisciplinary Meeting on Bioanalysis

Strany od

252

Strany do

255

Strany počet

475

URL

BibTex

@misc{BUT111690,
  author="Helena {Hudečková} and Petra {Šupinová} and Libor {Babák}",
  title="Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread",
  booktitle="CECE 2014 11th International Interdisciplinary Meeting on Bioanalysis",
  year="2014",
  pages="252--255",
  address="Ústav analytické chemie AV ČR, v. v. i.",
  isbn="978-80-904959-2-0",
  url="http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf",
  note="abstract"
}