Publication detail

Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells

POLJAKOVÁ, J. GROH, T. OMANA GUDINO, Ž. HRABĚTA, J. BOŘEK-DOHALSKÁ, L. KIZEK, R. DOKTOROVÁ, H. ECKSCHLAGER, T. STIBOROVÁ, M.

Original Title

Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells

Type

journal article in Web of Science

Language

English

Original Abstract

Cells of solid malignancies generally adapt to entire lack of oxygen. Hypoxia induces the expression of several genes, which allows the cells to survive. For DNA transcription, it is necessary that DNA structure is loosened. In addition to structural characteristics of DNA, its epigenetic alterations influence a proper DNA transcription. Since histones play a key role in epigenetics, changes in expression levels of acetylated histones H3 and H4 as well as of hypoxia-inducible factor-1a (HIF-1a) in human neuroblastoma cell lines cultivated under standard or hypoxic conditions (1% O2) were investigated. Moreover, the effect of hypoxia on the expression of two transcription factors, c-Myc and N-myc, was studied. Hypoxic stress increased levels of acetylated histones H3 and H4 in UKF-NB-3 and UKF-NB-4 neuroblastoma cells with N-myc amplification, whereas almost no changes in acetylation of these histones were found in an SK-N-AS neuroblastoma cell line, the line with diploid N-myc status. An increase in histone H4 acetylation caused by hypoxia in UKF-NB-3 and UKF-NB-4 corresponds to increased levels of N-myc transcription factor in these cells.

Keywords

hypoxia, neuroblastoma, histone acetylation, N-myc expression

Authors

POLJAKOVÁ, J.; GROH, T.; OMANA GUDINO, Ž.; HRABĚTA, J.; BOŘEK-DOHALSKÁ, L.; KIZEK, R.; DOKTOROVÁ, H.; ECKSCHLAGER, T.; STIBOROVÁ, M.

RIV year

2014

Released

1. 4. 2014

ISBN

1021-335X

Periodical

ONCOLOGY REPORTS

Year of study

31

Number

4

State

Hellenic Republic

Pages from

1928

Pages to

1934

Pages count

7

BibTex

@article{BUT112439,
  author="Jitka {Poljaková} and Tomáš {Groh} and Žaneta {Omana Gudino} and Jan {Hraběta} and Lucie {Bořek-Dohalská} and René {Kizek} and Helena {Doktorová} and Tomáš {Eckschlager} and Marie {Stiborová}",
  title="Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells",
  journal="ONCOLOGY REPORTS",
  year="2014",
  volume="31",
  number="4",
  pages="1928--1934",
  doi="10.3892/or.2014.2999",
  issn="1021-335X"
}