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POLJAKOVÁ, J. GROH, T. OMANA GUDINO, Ž. HRABĚTA, J. BOŘEK-DOHALSKÁ, L. KIZEK, R. DOKTOROVÁ, H. ECKSCHLAGER, T. STIBOROVÁ, M.
Originální název
Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Cells of solid malignancies generally adapt to entire lack of oxygen. Hypoxia induces the expression of several genes, which allows the cells to survive. For DNA transcription, it is necessary that DNA structure is loosened. In addition to structural characteristics of DNA, its epigenetic alterations influence a proper DNA transcription. Since histones play a key role in epigenetics, changes in expression levels of acetylated histones H3 and H4 as well as of hypoxia-inducible factor-1a (HIF-1a) in human neuroblastoma cell lines cultivated under standard or hypoxic conditions (1% O2) were investigated. Moreover, the effect of hypoxia on the expression of two transcription factors, c-Myc and N-myc, was studied. Hypoxic stress increased levels of acetylated histones H3 and H4 in UKF-NB-3 and UKF-NB-4 neuroblastoma cells with N-myc amplification, whereas almost no changes in acetylation of these histones were found in an SK-N-AS neuroblastoma cell line, the line with diploid N-myc status. An increase in histone H4 acetylation caused by hypoxia in UKF-NB-3 and UKF-NB-4 corresponds to increased levels of N-myc transcription factor in these cells.
Klíčová slova
hypoxia, neuroblastoma, histone acetylation, N-myc expression
Autoři
POLJAKOVÁ, J.; GROH, T.; OMANA GUDINO, Ž.; HRABĚTA, J.; BOŘEK-DOHALSKÁ, L.; KIZEK, R.; DOKTOROVÁ, H.; ECKSCHLAGER, T.; STIBOROVÁ, M.
Rok RIV
2014
Vydáno
1. 4. 2014
ISSN
1021-335X
Periodikum
ONCOLOGY REPORTS
Ročník
31
Číslo
4
Stát
Řecká republika
Strany od
1928
Strany do
1934
Strany počet
7
BibTex
@article{BUT112439, author="Jitka {Poljaková} and Tomáš {Groh} and Žaneta {Omana Gudino} and Jan {Hraběta} and Lucie {Bořek-Dohalská} and René {Kizek} and Helena {Doktorová} and Tomáš {Eckschlager} and Marie {Stiborová}", title="Hypoxia-mediated histone acetylation and expression of N-myc transcription factor dictate aggressiveness of neuroblastoma cells", journal="ONCOLOGY REPORTS", year="2014", volume="31", number="4", pages="1928--1934", doi="10.3892/or.2014.2999", issn="1021-335X" }