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BALVAN, J. KŘÍŽOVÁ, A. GUMULEC, J. RAUDENSKÁ, M. SLÁDEK, Z. SEDLÁČKOVÁ, M. BABULA, P. SZTALMACHOVÁ, M. KIZEK, R. CHMELÍK, R. MASAŘÍK, M.
Original Title
Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
Type
journal article in Web of Science
Language
English
Original Abstract
Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection Multimodal holographic microscopy (MHM). The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.
Keywords
Multimodal holographic microscopy; apoptosis; oncosis; necrosis; cell death; plumbagin; flow cytometry; transmission electron microscopy
Authors
BALVAN, J.; KŘÍŽOVÁ, A.; GUMULEC, J.; RAUDENSKÁ, M.; SLÁDEK, Z.; SEDLÁČKOVÁ, M.; BABULA, P.; SZTALMACHOVÁ, M.; KIZEK, R.; CHMELÍK, R.; MASAŘÍK, M.
RIV year
2015
Released
24. 3. 2015
Publisher
PLOS
ISBN
1932-6203
Periodical
PLOS ONE
Year of study
10
Number
3
State
United States of America
Pages from
1
Pages to
16
Pages count
URL
https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674
Full text in the Digital Library
http://hdl.handle.net/11012/193259
BibTex
@article{BUT113695, author="Jan {Balvan} and Aneta {Křížová} and Jaromír {Gumulec} and Martina {Raudenská} and Zbyšek {Sládek} and Miroslava {Sedláčková} and Petr {Babula} and Markéta {Svobodová} and René {Kizek} and Radim {Chmelík} and Michal {Masařík}", title="Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis", journal="PLOS ONE", year="2015", volume="10", number="3", pages="1--16", doi="10.1371/journal.pone.0121674", issn="1932-6203", url="https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674" }