Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
BALVAN, J. KŘÍŽOVÁ, A. GUMULEC, J. RAUDENSKÁ, M. SLÁDEK, Z. SEDLÁČKOVÁ, M. BABULA, P. SZTALMACHOVÁ, M. KIZEK, R. CHMELÍK, R. MASAŘÍK, M.
Originální název
Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection Multimodal holographic microscopy (MHM). The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.
Klíčová slova
Multimodal holographic microscopy; apoptosis; oncosis; necrosis; cell death; plumbagin; flow cytometry; transmission electron microscopy
Autoři
BALVAN, J.; KŘÍŽOVÁ, A.; GUMULEC, J.; RAUDENSKÁ, M.; SLÁDEK, Z.; SEDLÁČKOVÁ, M.; BABULA, P.; SZTALMACHOVÁ, M.; KIZEK, R.; CHMELÍK, R.; MASAŘÍK, M.
Rok RIV
2015
Vydáno
24. 3. 2015
Nakladatel
PLOS
ISSN
1932-6203
Periodikum
PLOS ONE
Ročník
10
Číslo
3
Stát
Spojené státy americké
Strany od
1
Strany do
16
Strany počet
URL
https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674
Plný text v Digitální knihovně
http://hdl.handle.net/11012/193259
BibTex
@article{BUT113695, author="Jan {Balvan} and Aneta {Křížová} and Jaromír {Gumulec} and Martina {Raudenská} and Zbyšek {Sládek} and Miroslava {Sedláčková} and Petr {Babula} and Markéta {Svobodová} and René {Kizek} and Radim {Chmelík} and Michal {Masařík}", title="Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis", journal="PLOS ONE", year="2015", volume="10", number="3", pages="1--16", doi="10.1371/journal.pone.0121674", issn="1932-6203", url="https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674" }