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ALTMAYER, M. MANZ, A. NEUŽIL, P.
Original Title
Microfluidic Superheating for Peptide Sequence Elucidation
Type
journal article in Web of Science
Language
English
Original Abstract
Herein, we introduce microfluidic superheating as a new method for peptide fragmentation prior to mass spectrometric analysis. The superheating conditions were found to be stable up to 240 C for more than 30 min without elevated pressure or boiling of the aqueous sample. As proof of principle, we exposed the peptides ACTH1-10 and OVA257-264 to various superheating conditions, causing different degrees of decomposition. Optimized superheating conditions resulted in the entire peptide ladder sequence of the y-ions, allowing the amino acid sequence to be deduced from a single-stage mass spectrum. Thus, obtaining information in the same quality as from tandem mass spectrometry can be achieved by a single superheating step.
Keywords
superheating, peptides, hydrolysis
Authors
ALTMAYER, M.; MANZ, A.; NEUŽIL, P.
RIV year
2015
Released
2. 6. 2015
ISBN
0003-2700
Periodical
ANALYTICAL CHEMISTRY
Year of study
87
Number
12
State
United States of America
Pages from
5997
Pages to
6003
Pages count
7
BibTex
@article{BUT115137, author="Matthias O. {Altmayer} and Andreas {Manz} and Pavel {Neužil}", title="Microfluidic Superheating for Peptide Sequence Elucidation", journal="ANALYTICAL CHEMISTRY", year="2015", volume="87", number="12", pages="5997--6003", doi="10.1021/acs.analchem.5b00189", issn="0003-2700" }