Detail publikace

Microfluidic Superheating for Peptide Sequence Elucidation

ALTMAYER, M. MANZ, A. NEUŽIL, P.

Originální název

Microfluidic Superheating for Peptide Sequence Elucidation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Herein, we introduce microfluidic superheating as a new method for peptide fragmentation prior to mass spectrometric analysis. The superheating conditions were found to be stable up to 240 C for more than 30 min without elevated pressure or boiling of the aqueous sample. As proof of principle, we exposed the peptides ACTH1-10 and OVA257-264 to various superheating conditions, causing different degrees of decomposition. Optimized superheating conditions resulted in the entire peptide ladder sequence of the y-ions, allowing the amino acid sequence to be deduced from a single-stage mass spectrum. Thus, obtaining information in the same quality as from tandem mass spectrometry can be achieved by a single superheating step.

Klíčová slova

superheating, peptides, hydrolysis

Autoři

ALTMAYER, M.; MANZ, A.; NEUŽIL, P.

Rok RIV

2015

Vydáno

2. 6. 2015

ISSN

0003-2700

Periodikum

ANALYTICAL CHEMISTRY

Ročník

87

Číslo

12

Stát

Spojené státy americké

Strany od

5997

Strany do

6003

Strany počet

7

BibTex

@article{BUT115137,
  author="Matthias O. {Altmayer} and Andreas {Manz} and Pavel {Neužil}",
  title="Microfluidic Superheating for Peptide Sequence Elucidation",
  journal="ANALYTICAL CHEMISTRY",
  year="2015",
  volume="87",
  number="12",
  pages="5997--6003",
  doi="10.1021/acs.analchem.5b00189",
  issn="0003-2700"
}