Publication detail

Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR

HRUDÍKOVÁ, R. PEPELIAEV, S.

Original Title

Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR

Type

abstract

Language

English

Original Abstract

The whole genome expression analysis of Streptococcus equi subsp. zooepidemicus strain ATCC 35246 was performed using DNA microarrays. According to the stability of gene expression 10 genes including gyrA and gyrB were chosen as potential reference genes. Further analysis of expression stability of selected genes was done by real-time PCR. Ct values from PCR analysis were processed in Microsoft Excel and our conclusions were additionally confirmed by GeNorm normalization. Reference gene was chosen

Keywords

Reference genes, real-time PCR, whole genome expression

Authors

HRUDÍKOVÁ, R. PEPELIAEV, S.

Released

23. 4. 2015

Publisher

Masaryk University

Location

Brno

ISBN

978-80-210-7825-3

Book

CEITEC PhD Retreat, Book of Abstracts

Pages from

49

Pages to

49

Pages count

1

BibTex

@misc{BUT115656,
  author="Radka {Hrudíková}",
  title="Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR",
  booktitle="CEITEC PhD Retreat, Book of Abstracts",
  year="2015",
  pages="49--49",
  publisher="Masaryk University",
  address="Brno",
  isbn="978-80-210-7825-3",
  note="abstract"
}