Publication detail

Electrochemical and Spectral Behavior of MicroRNA (miR34a5p)

VEČEŘOVÁ, A. HUDCOVÁ, K. PILAŘOVÁ, I. MASAŘÍK, M. TRNKOVÁ, L.

Original Title

Electrochemical and Spectral Behavior of MicroRNA (miR34a5p)

Type

conference paper

Language

English

Original Abstract

MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA? miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3–6.

Keywords

miR-34a-5p; Human cancer; Voltammetry; Circular dichroic spectroscopy; Mercury electrode

Authors

VEČEŘOVÁ, A.; HUDCOVÁ, K.; PILAŘOVÁ, I.; MASAŘÍK, M.; TRNKOVÁ, L.

RIV year

2015

Released

9. 11. 2015

ISBN

978-80-905221-3-8

Book

Proceeedings of XXXV International Conference on Modern Electrochemical Methods

Pages from

265

Pages to

268

Pages count

6

BibTex

@inproceedings{BUT118731,
  author="Aneta {Večeřová} and Kristýna {Hudcová} and Iveta {Pilařová} and Michal {Masařík} and Libuše {Trnková}",
  title="Electrochemical and Spectral Behavior of MicroRNA (miR34a5p)",
  booktitle="Proceeedings of XXXV International Conference on Modern Electrochemical Methods",
  year="2015",
  pages="265--268",
  doi="10.1007/s00706-015-1600-y",
  isbn="978-80-905221-3-8"
}