Detail publikace

Electrochemical and Spectral Behavior of MicroRNA (miR34a5p)

VEČEŘOVÁ, A. HUDCOVÁ, K. PILAŘOVÁ, I. MASAŘÍK, M. TRNKOVÁ, L.

Originální název

Electrochemical and Spectral Behavior of MicroRNA (miR34a5p)

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA? miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3–6.

Klíčová slova

miR-34a-5p; Human cancer; Voltammetry; Circular dichroic spectroscopy; Mercury electrode

Autoři

VEČEŘOVÁ, A.; HUDCOVÁ, K.; PILAŘOVÁ, I.; MASAŘÍK, M.; TRNKOVÁ, L.

Rok RIV

2015

Vydáno

9. 11. 2015

ISBN

978-80-905221-3-8

Kniha

Proceeedings of XXXV International Conference on Modern Electrochemical Methods

Strany od

265

Strany do

268

Strany počet

6

BibTex

@inproceedings{BUT118731,
  author="Aneta {Večeřová} and Kristýna {Hudcová} and Iveta {Pilařová} and Michal {Masařík} and Libuše {Trnková}",
  title="Electrochemical and Spectral Behavior of MicroRNA (miR34a5p)",
  booktitle="Proceeedings of XXXV International Conference on Modern Electrochemical Methods",
  year="2015",
  pages="265--268",
  doi="10.1007/s00706-015-1600-y",
  isbn="978-80-905221-3-8"
}