Publication detail

Handheld Real-Time PCR Device

AHRBERG, C. ILIC, B. MANZ, A. NEUŽIL, P.

Original Title

Handheld Real-Time PCR Device

Type

journal article in Web of Science

Language

English

Original Abstract

Here we report one of the smallest real-time polymerase chain reaction (PCR) system up to date with approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in form of virtual reaction chambers (VRC) where a ≈ 200 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate PCR performance. The standard curve slope was (-3.02 ± 0.16) cycles at threshold per decade (mean ± standard deviation) corresponding to an amplification efficiency of (0.91 ± 0.05) per cycle (mean ± standard deviation). The PCR device was capable of detecting a single deoxyribonucleic acid (DNA) copy. These results further suggest that our handheld PCR device may have broad, technologically-relevant applications extending to rapid detection of infectious diseases in small clinics.

Keywords

real-time PCR; palm-size; portable; standard curve; reproducibility

Authors

AHRBERG, C.; ILIC, B.; MANZ, A.; NEUŽIL, P.

Released

4. 1. 2016

Publisher

Royal Society of Chemistry

ISBN

1473-0197

Periodical

LAB ON A CHIP

Year of study

16

Number

3

State

United Kingdom of Great Britain and Northern Ireland

Pages from

586

Pages to

592

Pages count

7

URL

http://pubs.rsc.org/is/content/articlelanding/2015/lc/c5lc01415h#!divAbstract

Full text in the Digital Library

http://hdl.handle.net/11012/61768

BibTex

@article{BUT120126,
  author="Christian D. {Ahrberg} and Bojan Robert {Ilic} and Andreas {Manz} and Pavel {Neužil}",
  title="Handheld Real-Time PCR Device",
  journal="LAB ON A CHIP",
  year="2016",
  volume="16",
  number="3",
  pages="586--592",
  doi="10.1039/c5lc01415h",
  issn="1473-0197",
  url="http://pubs.rsc.org/is/content/articlelanding/2015/lc/c5lc01415h#!divAbstract"
}