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Detail publikace
AHRBERG, C. ILIC, B. MANZ, A. NEUŽIL, P.
Originální název
Handheld Real-Time PCR Device
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Here we report one of the smallest real-time polymerase chain reaction (PCR) system up to date with approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in form of virtual reaction chambers (VRC) where a ≈ 200 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate PCR performance. The standard curve slope was (-3.02 ± 0.16) cycles at threshold per decade (mean ± standard deviation) corresponding to an amplification efficiency of (0.91 ± 0.05) per cycle (mean ± standard deviation). The PCR device was capable of detecting a single deoxyribonucleic acid (DNA) copy. These results further suggest that our handheld PCR device may have broad, technologically-relevant applications extending to rapid detection of infectious diseases in small clinics.
Klíčová slova
real-time PCR; palm-size; portable; standard curve; reproducibility
Autoři
AHRBERG, C.; ILIC, B.; MANZ, A.; NEUŽIL, P.
Vydáno
4. 1. 2016
Nakladatel
Royal Society of Chemistry
ISSN
1473-0197
Periodikum
LAB ON A CHIP
Ročník
16
Číslo
3
Stát
Spojené království Velké Británie a Severního Irska
Strany od
586
Strany do
592
Strany počet
7
URL
http://pubs.rsc.org/is/content/articlelanding/2015/lc/c5lc01415h#!divAbstract
Plný text v Digitální knihovně
http://hdl.handle.net/11012/61768
BibTex
@article{BUT120126, author="Christian D. {Ahrberg} and Bojan Robert {Ilic} and Andreas {Manz} and Pavel {Neužil}", title="Handheld Real-Time PCR Device", journal="LAB ON A CHIP", year="2016", volume="16", number="3", pages="586--592", doi="10.1039/c5lc01415h", issn="1473-0197", url="http://pubs.rsc.org/is/content/articlelanding/2015/lc/c5lc01415h#!divAbstract" }