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KŘÍŽKOVÁ, S. DOSTÁLOVÁ, S. MICHÁLEK, P. NEJDL, L. KOMÍNKOVÁ, M. MILOSAVLJEVIĆ, V. MOULICK, A. VACULOVIČOVÁ, M. KOPEL, P. ADAM, V. KÍZEK, R.
Original Title
SDS-PAGE as a tool for hydrodynamic diameter-dependent separation of quantum dots
Type
journal article in Web of Science
Language
English
Original Abstract
The purpose of this study was to test quantum dots (QDs) separation by native and Tris-Glycine SDS-PAGE according to the protocols commonly used for protein analyses. To study the electrophoretic behaviour of quantum dots, ten samples of previously synthesized CdTe QDs stabilized with mercaptosuccinic acid (MSA) were used. Prior to electrophoresis the hydrodynamic diameters of QDs and zeta potentials were determined, as well as the fluorescence properties and stability of QDs in the running buffers. After verification of QDs stability and separation in native polyacrylamide gel, SDS-PAGE in gradient 4-20 % polyacrylamide gel was performed. Under UV irradiation a colour-dependent separation of QDs was observed, which was consistent with their hydrodynamic diameter distribution. The electrophoretic conditions were further optimized with respect to achieving the optimal colour separation, fluorescence stability and to minimize the time of analysis. Based on the results obtained, for further work 15 % polyacrylamide gels with SDS were used and the times (30-60 min) and voltage (100-150 V) used for separation were optimized. Under the optimal separation conditions (30 min, 100 V) the addition of MSA in the concentration range 0-4 mM was used to improve visualization of QDs with diameters in the range from 7 +/- A 2 to 4 +/- A 2 nm.
Keywords
Quantum dots; SDS-PAGE; Electrophoresis; Separation; Hydrodynamic diameter-dependent
Authors
KŘÍŽKOVÁ, S.; DOSTÁLOVÁ, S.; MICHÁLEK, P.; NEJDL, L.; KOMÍNKOVÁ, M.; MILOSAVLJEVIĆ, V.; MOULICK, A.; VACULOVIČOVÁ, M.; KOPEL, P.; ADAM, V.; KÍZEK, R.
RIV year
2015
Released
1. 6. 2015
ISBN
0009-5893
Periodical
CHROMATOGRAPHIA
Year of study
78
Number
11-12
State
Federal Republic of Germany
Pages from
785
Pages to
793
Pages count
9
BibTex
@article{BUT123334, author="Soňa {Křížková} and Simona {Dostálová} and Petr {Michálek} and Lukáš {Nejdl} and Markéta {Komínková} and Vedran {Milosavljević} and Amitava {Moulick} and Markéta {Vaculovičová} and Pavel {Kopel} and Vojtěch {Adam} and René {Kízek}", title="SDS-PAGE as a tool for hydrodynamic diameter-dependent separation of quantum dots", journal="CHROMATOGRAPHIA", year="2015", volume="78", number="11-12", pages="785--793", doi="10.1007/s10337-015-2893-z", issn="0009-5893" }