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KŘÍŽKOVÁ, S. DOSTÁLOVÁ, S. MICHÁLEK, P. NEJDL, L. KOMÍNKOVÁ, M. MILOSAVLJEVIĆ, V. MOULICK, A. VACULOVIČOVÁ, M. KOPEL, P. ADAM, V. KÍZEK, R.
Originální název
SDS-PAGE as a tool for hydrodynamic diameter-dependent separation of quantum dots
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The purpose of this study was to test quantum dots (QDs) separation by native and Tris-Glycine SDS-PAGE according to the protocols commonly used for protein analyses. To study the electrophoretic behaviour of quantum dots, ten samples of previously synthesized CdTe QDs stabilized with mercaptosuccinic acid (MSA) were used. Prior to electrophoresis the hydrodynamic diameters of QDs and zeta potentials were determined, as well as the fluorescence properties and stability of QDs in the running buffers. After verification of QDs stability and separation in native polyacrylamide gel, SDS-PAGE in gradient 4-20 % polyacrylamide gel was performed. Under UV irradiation a colour-dependent separation of QDs was observed, which was consistent with their hydrodynamic diameter distribution. The electrophoretic conditions were further optimized with respect to achieving the optimal colour separation, fluorescence stability and to minimize the time of analysis. Based on the results obtained, for further work 15 % polyacrylamide gels with SDS were used and the times (30-60 min) and voltage (100-150 V) used for separation were optimized. Under the optimal separation conditions (30 min, 100 V) the addition of MSA in the concentration range 0-4 mM was used to improve visualization of QDs with diameters in the range from 7 +/- A 2 to 4 +/- A 2 nm.
Klíčová slova
Quantum dots; SDS-PAGE; Electrophoresis; Separation; Hydrodynamic diameter-dependent
Autoři
KŘÍŽKOVÁ, S.; DOSTÁLOVÁ, S.; MICHÁLEK, P.; NEJDL, L.; KOMÍNKOVÁ, M.; MILOSAVLJEVIĆ, V.; MOULICK, A.; VACULOVIČOVÁ, M.; KOPEL, P.; ADAM, V.; KÍZEK, R.
Rok RIV
2015
Vydáno
1. 6. 2015
ISSN
0009-5893
Periodikum
CHROMATOGRAPHIA
Ročník
78
Číslo
11-12
Stát
Spolková republika Německo
Strany od
785
Strany do
793
Strany počet
9
BibTex
@article{BUT123334, author="Soňa {Křížková} and Simona {Dostálová} and Petr {Michálek} and Lukáš {Nejdl} and Markéta {Komínková} and Vedran {Milosavljević} and Amitava {Moulick} and Markéta {Vaculovičová} and Pavel {Kopel} and Vojtěch {Adam} and René {Kízek}", title="SDS-PAGE as a tool for hydrodynamic diameter-dependent separation of quantum dots", journal="CHROMATOGRAPHIA", year="2015", volume="78", number="11-12", pages="785--793", doi="10.1007/s10337-015-2893-z", issn="0009-5893" }