Publication detail

Relation of Exposure to Amino Acids Involved in Sarcosine Metabolic Pathway on Behavior of Non-Tumor and Malignant Prostatic Cell Lines

HEGER, Z. GUMULEC, J. CERNEI, N. POLANSKÁ, H. RAUDENSKÁ, M. MASAŘÍK, M. ECKSCHLAGER, T. STIBOROVÁ, M. ADAM, V. KIZEK, R.

Original Title

Relation of Exposure to Amino Acids Involved in Sarcosine Metabolic Pathway on Behavior of Non-Tumor and Malignant Prostatic Cell Lines

Type

journal article in Web of Science

Language

English

Original Abstract

Sarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. We focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using realtime cell based assay). The effect of supplementation on expression of glycine- N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward’s method, factorial ANOVA and principal component analysis (P<0.05). The highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found.

Keywords

cancer metabolism; dimethylglycine; folate; glycine; sarcosine pathway; prostate cancer

Authors

HEGER, Z.; GUMULEC, J.; CERNEI, N.; POLANSKÁ, H.; RAUDENSKÁ, M.; MASAŘÍK, M.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ADAM, V.; KIZEK, R.

Released

15. 5. 2016

ISBN

0270-4137

Periodical

PROSTATE

Year of study

76

Number

7

State

United States of America

Pages from

679

Pages to

690

Pages count

12

BibTex

@article{BUT128497,
  author="Zbyněk {Heger} and Jaromír {Gumulec} and Natalia Vladimirovna {Cernei} and Hana {Polanská} and Martina {Raudenská} and Michal {Masařík} and Tomáš {Eckschlager} and Marie {Stiborová} and Vojtěch {Adam} and René {Kizek}",
  title="Relation of Exposure to Amino Acids Involved in Sarcosine Metabolic Pathway on Behavior of Non-Tumor and Malignant Prostatic Cell Lines",
  journal="PROSTATE",
  year="2016",
  volume="76",
  number="7",
  pages="679--690",
  doi="10.1002/pros.23159",
  issn="0270-4137"
}