Detail publikace

Relation of Exposure to Amino Acids Involved in Sarcosine Metabolic Pathway on Behavior of Non-Tumor and Malignant Prostatic Cell Lines

HEGER, Z. GUMULEC, J. CERNEI, N. POLANSKÁ, H. RAUDENSKÁ, M. MASAŘÍK, M. ECKSCHLAGER, T. STIBOROVÁ, M. ADAM, V. KIZEK, R.

Originální název

Relation of Exposure to Amino Acids Involved in Sarcosine Metabolic Pathway on Behavior of Non-Tumor and Malignant Prostatic Cell Lines

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Sarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. We focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using realtime cell based assay). The effect of supplementation on expression of glycine- N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward’s method, factorial ANOVA and principal component analysis (P<0.05). The highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found.

Klíčová slova

cancer metabolism; dimethylglycine; folate; glycine; sarcosine pathway; prostate cancer

Autoři

HEGER, Z.; GUMULEC, J.; CERNEI, N.; POLANSKÁ, H.; RAUDENSKÁ, M.; MASAŘÍK, M.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ADAM, V.; KIZEK, R.

Vydáno

15. 5. 2016

ISSN

0270-4137

Periodikum

PROSTATE

Ročník

76

Číslo

7

Stát

Spojené státy americké

Strany od

679

Strany do

690

Strany počet

12

BibTex

@article{BUT128497,
  author="Zbyněk {Heger} and Jaromír {Gumulec} and Natalia Vladimirovna {Cernei} and Hana {Polanská} and Martina {Raudenská} and Michal {Masařík} and Tomáš {Eckschlager} and Marie {Stiborová} and Vojtěch {Adam} and René {Kizek}",
  title="Relation of Exposure to Amino Acids Involved in Sarcosine Metabolic Pathway on Behavior of Non-Tumor and Malignant Prostatic Cell Lines",
  journal="PROSTATE",
  year="2016",
  volume="76",
  number="7",
  pages="679--690",
  doi="10.1002/pros.23159",
  issn="0270-4137"
}