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DVOŘÁK, P. KURUMBANG, N. BENDL, J. BREZOVSKÝ, J. PROKOP, Z. DAMBORSKÝ, J.
Original Title
Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization
Type
journal article in Web of Science
Language
English
Original Abstract
Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts' stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes.
Keywords
biocatalysis, biotransformation, kinetic modelling
Authors
DVOŘÁK, P.; KURUMBANG, N.; BENDL, J.; BREZOVSKÝ, J.; PROKOP, Z.; DAMBORSKÝ, J.
RIV year
2014
Released
2. 4. 2014
ISBN
1439-4227
Periodical
CHEMBIOCHEM
Year of study
15
Number
13
State
Federal Republic of Germany
Pages from
1891
Pages to
1895
Pages count
5
URL
http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402265/full
BibTex
@article{BUT133484, author="Pavel {Dvořák} and Nagendra {Kurumbang} and Jaroslav {Bendl} and Jan {Brezovský} and Zbyněk {Prokop} and Jiří {Damborský}", title="Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization", journal="CHEMBIOCHEM", year="2014", volume="15", number="13", pages="1891--1895", doi="10.1002/cbic.201402265", issn="1439-4227", url="http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402265/full" }