Publication detail

Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization

DVOŘÁK, P. KURUMBANG, N. BENDL, J. BREZOVSKÝ, J. PROKOP, Z. DAMBORSKÝ, J.

Original Title

Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization

Type

journal article in Web of Science

Language

English

Original Abstract

Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts' stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes.

Keywords

biocatalysis, biotransformation, kinetic modelling

Authors

DVOŘÁK, P.; KURUMBANG, N.; BENDL, J.; BREZOVSKÝ, J.; PROKOP, Z.; DAMBORSKÝ, J.

RIV year

2014

Released

2. 4. 2014

ISBN

1439-4227

Periodical

CHEMBIOCHEM

Year of study

15

Number

13

State

Federal Republic of Germany

Pages from

1891

Pages to

1895

Pages count

5

URL

http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402265/full

BibTex

@article{BUT133484,
  author="Pavel {Dvořák} and Nagendra {Kurumbang} and Jaroslav {Bendl} and Jan {Brezovský} and Zbyněk {Prokop} and Jiří {Damborský}",
  title="Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization",
  journal="CHEMBIOCHEM",
  year="2014",
  volume="15",
  number="13",
  pages="1891--1895",
  doi="10.1002/cbic.201402265",
  issn="1439-4227",
  url="http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402265/full"
}

Documents

Dvorak_ChemBioChem_final.doc
Dvorak_ChemBioChem_supplement.pdf
Dvorak_ChemBioChem_publkovane.pdf