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DVOŘÁK, P. KURUMBANG, N. BENDL, J. BREZOVSKÝ, J. PROKOP, Z. DAMBORSKÝ, J.
Originální název
Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts' stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes.
Klíčová slova
biocatalysis, biotransformation, kinetic modelling
Autoři
DVOŘÁK, P.; KURUMBANG, N.; BENDL, J.; BREZOVSKÝ, J.; PROKOP, Z.; DAMBORSKÝ, J.
Rok RIV
2014
Vydáno
2. 4. 2014
ISSN
1439-4227
Periodikum
CHEMBIOCHEM
Ročník
15
Číslo
13
Stát
Spolková republika Německo
Strany od
1891
Strany do
1895
Strany počet
5
URL
http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402265/full
BibTex
@article{BUT133484, author="Pavel {Dvořák} and Nagendra {Kurumbang} and Jaroslav {Bendl} and Jan {Brezovský} and Zbyněk {Prokop} and Jiří {Damborský}", title="Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization", journal="CHEMBIOCHEM", year="2014", volume="15", number="13", pages="1891--1895", doi="10.1002/cbic.201402265", issn="1439-4227", url="http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402265/full" }