Přístupnostní navigace
E-application
Search Search Close
Publication detail
MILOSAVLJEVIĆ, V. JELÍNKOVÁ, P. JIMENEZ JIMENEZ, A. MOULICK, A. HADDAD, Y. BUCHTELOVÁ, H. KŘÍŽKOVÁ, S. HEGER, Z. KALINA, L. RICHTERA, L. KOPEL, P. ADAM, V.
Original Title
Alternative synthesis route of biocompatible polyvinylpyrrolidone nanoparticles and their effect on pathogenic microorganisms
Type
journal article in Web of Science
Language
English
Original Abstract
Herein we describe a novel alternative synthesis route of polyvinylpyrrolidone nanoparticles using salting-out method at a temperature close to polyvinylpyrrolidone decomposition. At elevated temperatures, the stability of polyvinylpyrrolidone decreases and the opening of pyrrolidone ring fractions occurs. This leads to cross-linking process, where separate units of polyvinylpyrrolidone interact among themselves and rearrange to form nanoparticles. The formation/stability of these nanoparticles was confirmed by transmission electron microscopy, Xray photoelectron spectroscopy, mass spectrometry, infrared spectroscopy, and spectrophotometry. The obtained nanoparticles possess exceptional biocompatibility. No toxicity and, genotoxicity was found in normal human prostate epithelium cells (PNT1A) together with their high hemocompatibility. The antimicrobial effects of polprinylpyrrolidone nanoparticles, were tested on bacterial strains isolated from the wounds of patients suffering from hard-to-heal infections. Molecular analysis (VCR) confirmed that the treatment can induce the regulation of stress-related survival genes. Our results strongly suggest that the polyvinylpyrrolidone nanoparticles have great potential to be developed into a novel antibacterial compound.
Keywords
antibacterial; cross-linking; polyvinylpyrrolidone nanoparticles; salting-out; toxicity
Authors
MILOSAVLJEVIĆ, V.; JELÍNKOVÁ, P.; JIMENEZ JIMENEZ, A.; MOULICK, A.; HADDAD, Y.; BUCHTELOVÁ, H.; KŘÍŽKOVÁ, S.; HEGER, Z.; KALINA, L.; RICHTERA, L.; KOPEL, P.; ADAM, V.
Released
3. 1. 2017
ISBN
1543-8384
Periodical
MOLECULAR PHARMACEUTICS
Year of study
14
Number
1
State
United States of America
Pages from
221
Pages to
233
Pages count
13
URL
http://pubs.acs.org/doi/pdf/10.1021/acs.molpharmaceut.6b00807
BibTex
@article{BUT137482, author="Vedran {Milosavljević} and Pavlína {Jelínková} and Ana Maria {Jimenez Jimenez} and Amitava {Moulick} and Yazan Abdulmajeed Eyadh {Haddad} and Hana {Michálková} and Soňa {Křížková} and Zbyněk {Heger} and Lukáš {Kalina} and Lukáš {Richtera} and Pavel {Kopel} and Vojtěch {Adam}", title="Alternative synthesis route of biocompatible polyvinylpyrrolidone nanoparticles and their effect on pathogenic microorganisms", journal="MOLECULAR PHARMACEUTICS", year="2017", volume="14", number="1", pages="221--233", doi="10.1021/acs.molpharmaceut.6b00807", issn="1543-8384", url="http://pubs.acs.org/doi/pdf/10.1021/acs.molpharmaceut.6b00807" }