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MILOSAVLJEVIĆ, V. JELÍNKOVÁ, P. JIMENEZ JIMENEZ, A. MOULICK, A. HADDAD, Y. BUCHTELOVÁ, H. KŘÍŽKOVÁ, S. HEGER, Z. KALINA, L. RICHTERA, L. KOPEL, P. ADAM, V.
Originální název
Alternative synthesis route of biocompatible polyvinylpyrrolidone nanoparticles and their effect on pathogenic microorganisms
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Herein we describe a novel alternative synthesis route of polyvinylpyrrolidone nanoparticles using salting-out method at a temperature close to polyvinylpyrrolidone decomposition. At elevated temperatures, the stability of polyvinylpyrrolidone decreases and the opening of pyrrolidone ring fractions occurs. This leads to cross-linking process, where separate units of polyvinylpyrrolidone interact among themselves and rearrange to form nanoparticles. The formation/stability of these nanoparticles was confirmed by transmission electron microscopy, Xray photoelectron spectroscopy, mass spectrometry, infrared spectroscopy, and spectrophotometry. The obtained nanoparticles possess exceptional biocompatibility. No toxicity and, genotoxicity was found in normal human prostate epithelium cells (PNT1A) together with their high hemocompatibility. The antimicrobial effects of polprinylpyrrolidone nanoparticles, were tested on bacterial strains isolated from the wounds of patients suffering from hard-to-heal infections. Molecular analysis (VCR) confirmed that the treatment can induce the regulation of stress-related survival genes. Our results strongly suggest that the polyvinylpyrrolidone nanoparticles have great potential to be developed into a novel antibacterial compound.
Klíčová slova
antibacterial; cross-linking; polyvinylpyrrolidone nanoparticles; salting-out; toxicity
Autoři
MILOSAVLJEVIĆ, V.; JELÍNKOVÁ, P.; JIMENEZ JIMENEZ, A.; MOULICK, A.; HADDAD, Y.; BUCHTELOVÁ, H.; KŘÍŽKOVÁ, S.; HEGER, Z.; KALINA, L.; RICHTERA, L.; KOPEL, P.; ADAM, V.
Vydáno
3. 1. 2017
ISSN
1543-8384
Periodikum
MOLECULAR PHARMACEUTICS
Ročník
14
Číslo
1
Stát
Spojené státy americké
Strany od
221
Strany do
233
Strany počet
13
URL
http://pubs.acs.org/doi/pdf/10.1021/acs.molpharmaceut.6b00807
BibTex
@article{BUT137482, author="Vedran {Milosavljević} and Pavlína {Jelínková} and Ana Maria {Jimenez Jimenez} and Amitava {Moulick} and Yazan Abdulmajeed Eyadh {Haddad} and Hana {Michálková} and Soňa {Křížková} and Zbyněk {Heger} and Lukáš {Kalina} and Lukáš {Richtera} and Pavel {Kopel} and Vojtěch {Adam}", title="Alternative synthesis route of biocompatible polyvinylpyrrolidone nanoparticles and their effect on pathogenic microorganisms", journal="MOLECULAR PHARMACEUTICS", year="2017", volume="14", number="1", pages="221--233", doi="10.1021/acs.molpharmaceut.6b00807", issn="1543-8384", url="http://pubs.acs.org/doi/pdf/10.1021/acs.molpharmaceut.6b00807" }