Publication detail

Electrochemical and Optical Study of Metallothionein Interactions with Prion Proteins

CARDOVÁ, A. ADAM, P. MARIANI, S. RICHTERA, L. HEGER, Z. LABUDA, J. MINUNNI, M. ADAM, V.

Original Title

Electrochemical and Optical Study of Metallothionein Interactions with Prion Proteins

Type

journal article in Web of Science

Language

English

Original Abstract

The prion protein (PrPc) can be structurally shifted to its PrPsc isoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrPc and MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrPc, MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS). Electrochemical properties of PrP itself and its interactions with both the Cu(II) ions and MTs have been found. Based on the results obtained, we hypothesised the formation of the complex in molar ratio 2:1 (PrPc:MT). Surface plasmon resonance imaging (SPRi) was used as a control reference assay to further confirm results obtained by the electrochemical approach, such as the specific interactions between PrPc and MT-3.

Keywords

PrP; MT; Brain; Neurodegenerative disorders; Electrochemistry

Authors

CARDOVÁ, A.; ADAM, P.; MARIANI, S.; RICHTERA, L.; HEGER, Z.; LABUDA, J.; MINUNNI, M.; ADAM, V.

Released

5. 6. 2017

ISBN

0731-7085

Periodical

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Year of study

140

Number

1

State

Kingdom of the Netherlands

Pages from

355

Pages to

361

Pages count

7

BibTex

@article{BUT138241,
  author="Alžběta {Cardová} and Pavlína {Adam} and Stefano {Mariani} and Lukáš {Richtera} and Zbyněk {Heger} and Jan {Labuda} and Maria {Minunni} and Vojtěch {Adam}",
  title="Electrochemical and Optical Study of Metallothionein Interactions with Prion Proteins",
  journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS",
  year="2017",
  volume="140",
  number="1",
  pages="355--361",
  doi="10.1016/j.jpba.2017.03.044",
  issn="0731-7085"
}