Detail publikace

Electrochemical and Optical Study of Metallothionein Interactions with Prion Proteins

CARDOVÁ, A. ADAM, P. MARIANI, S. RICHTERA, L. HEGER, Z. LABUDA, J. MINUNNI, M. ADAM, V.

Originální název

Electrochemical and Optical Study of Metallothionein Interactions with Prion Proteins

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The prion protein (PrPc) can be structurally shifted to its PrPsc isoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrPc and MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrPc, MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS). Electrochemical properties of PrP itself and its interactions with both the Cu(II) ions and MTs have been found. Based on the results obtained, we hypothesised the formation of the complex in molar ratio 2:1 (PrPc:MT). Surface plasmon resonance imaging (SPRi) was used as a control reference assay to further confirm results obtained by the electrochemical approach, such as the specific interactions between PrPc and MT-3.

Klíčová slova

PrP; MT; Brain; Neurodegenerative disorders; Electrochemistry

Autoři

CARDOVÁ, A.; ADAM, P.; MARIANI, S.; RICHTERA, L.; HEGER, Z.; LABUDA, J.; MINUNNI, M.; ADAM, V.

Vydáno

5. 6. 2017

ISSN

0731-7085

Periodikum

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Ročník

140

Číslo

1

Stát

Nizozemsko

Strany od

355

Strany do

361

Strany počet

7

BibTex

@article{BUT138241,
  author="Alžběta {Cardová} and Pavlína {Adam} and Stefano {Mariani} and Lukáš {Richtera} and Zbyněk {Heger} and Jan {Labuda} and Maria {Minunni} and Vojtěch {Adam}",
  title="Electrochemical and Optical Study of Metallothionein Interactions with Prion Proteins",
  journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS",
  year="2017",
  volume="140",
  number="1",
  pages="355--361",
  doi="10.1016/j.jpba.2017.03.044",
  issn="0731-7085"
}