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CHUDOBOVA, D. ČÍHALOVÁ, K. GURÁŇ, R. DOSTÁLOVÁ, S. ŠMERKOVÁ, K. VESELÝ, R. GUMULEC, J. HEGER, Z. MASAŘÍK, M. ADAM, V. KIZEK, R.
Original Title
IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING
Type
conference paper
Language
English
Original Abstract
This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was <2.
Keywords
MALDI-TOF MS; bacterial infection; Sanger sequencing
Authors
CHUDOBOVA, D.; ČÍHALOVÁ, K.; GURÁŇ, R.; DOSTÁLOVÁ, S.; ŠMERKOVÁ, K.; VESELÝ, R.; GUMULEC, J.; HEGER, Z.; MASAŘÍK, M.; ADAM, V.; KIZEK, R.
Released
22. 10. 2014
ISBN
978-80-904959-2-0
Book
CECE 2014
Pages from
178
Pages to
181
Pages count
4
BibTex
@inproceedings{BUT142427, author="Dagmar {chudobova} and Kristýna {Číhalová} and Roman {Guráň} and Simona {Dostálová} and Kristýna {Šmerková} and Radek {Veselý} and Jaromír {Gumulec} and Zbyněk {Heger} and Michal {Masařík} and Vojtěch {Adam} and René {Kizek}", title="IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING", booktitle="CECE 2014", year="2014", pages="178--181", isbn="978-80-904959-2-0" }