Publication detail

IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING

CHUDOBOVA, D. ČÍHALOVÁ, K. GURÁŇ, R. DOSTÁLOVÁ, S. ŠMERKOVÁ, K. VESELÝ, R. GUMULEC, J. HEGER, Z. MASAŘÍK, M. ADAM, V. KIZEK, R.

Original Title

IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING

Type

conference paper

Language

English

Original Abstract

This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was <2.

Keywords

MALDI-TOF MS; bacterial infection; Sanger sequencing

Authors

CHUDOBOVA, D.; ČÍHALOVÁ, K.; GURÁŇ, R.; DOSTÁLOVÁ, S.; ŠMERKOVÁ, K.; VESELÝ, R.; GUMULEC, J.; HEGER, Z.; MASAŘÍK, M.; ADAM, V.; KIZEK, R.

Released

22. 10. 2014

ISBN

978-80-904959-2-0

Book

CECE 2014

Pages from

178

Pages to

181

Pages count

4

BibTex

@inproceedings{BUT142427,
  author="Dagmar {chudobova} and Kristýna {Číhalová} and Roman {Guráň} and Simona {Dostálová} and Kristýna {Šmerková} and Radek {Veselý} and Jaromír {Gumulec} and Zbyněk {Heger} and Michal {Masařík} and Vojtěch {Adam} and René {Kizek}",
  title="IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING",
  booktitle="CECE 2014",
  year="2014",
  pages="178--181",
  isbn="978-80-904959-2-0"
}