Detail publikace

IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING

CHUDOBOVA, D. ČÍHALOVÁ, K. GURÁŇ, R. DOSTÁLOVÁ, S. ŠMERKOVÁ, K. VESELÝ, R. GUMULEC, J. HEGER, Z. MASAŘÍK, M. ADAM, V. KIZEK, R.

Originální název

IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was <2.

Klíčová slova

MALDI-TOF MS; bacterial infection; Sanger sequencing

Autoři

CHUDOBOVA, D.; ČÍHALOVÁ, K.; GURÁŇ, R.; DOSTÁLOVÁ, S.; ŠMERKOVÁ, K.; VESELÝ, R.; GUMULEC, J.; HEGER, Z.; MASAŘÍK, M.; ADAM, V.; KIZEK, R.

Vydáno

22. 10. 2014

ISBN

978-80-904959-2-0

Kniha

CECE 2014

Strany od

178

Strany do

181

Strany počet

4

BibTex

@inproceedings{BUT142427,
  author="Dagmar {chudobova} and Kristýna {Číhalová} and Roman {Guráň} and Simona {Dostálová} and Kristýna {Šmerková} and Radek {Veselý} and Jaromír {Gumulec} and Zbyněk {Heger} and Michal {Masařík} and Vojtěch {Adam} and René {Kizek}",
  title="IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING",
  booktitle="CECE 2014",
  year="2014",
  pages="178--181",
  isbn="978-80-904959-2-0"
}