Publication detail

G-Quadruplexes as Sensing Probes

RUTTKAY-NEDECKÝ, B. KUDR, J. NEJDL, L. MAŠKOVÁ, D. KIZEK, R. ADAM, V.

Original Title

G-Quadruplexes as Sensing Probes

Type

journal article in Web of Science

Language

English

Original Abstract

Guanine-rich sequences of DNA are able to create tetrastranded structures known as G-quadruplexes; they are formed by the stacking of planar G-quartets composed of four guanines paired by Hoogsteen hydrogen bonding. G-quadruplexes act as ligands for metal ions and aptamers for various molecules. Interestingly, the G-quadruplexes form a complex with anionic porphyrin hemin and exhibit peroxidase-like activity. This review focuses on overview of sensing techniques based on G-quadruplex complexes with anionic porphyrins for detection of various analytes, including metal ions such as K+, Ca2+, Ag+, Hg2+, Cu2+, Pb2+, Sr2+, organic molecules, nucleic acids, and proteins. Principles of G-quadruplex-based detection methods involve DNA conformational change caused by the presence of analyte which leads to a decrease or an increase in peroxidase activity, fluorescence, or electrochemical signal of the used probe. The advantages of various detection techniques are also discussed.

Keywords

G-quadruplex; DNAzyme; hemin

Authors

RUTTKAY-NEDECKÝ, B.; KUDR, J.; NEJDL, L.; MAŠKOVÁ, D.; KIZEK, R.; ADAM, V.

Released

1. 12. 2013

Publisher

MDPI

ISBN

1420-3049

Periodical

MOLECULES

Year of study

18

Number

12

State

Swiss Confederation

Pages from

14760

Pages to

14779

Pages count

20

URL

https://www.mdpi.com/1420-3049/18/12/14760

Full text in the Digital Library

http://hdl.handle.net/11012/137244

BibTex

@article{BUT145126,
  author="Branislav {Ruttkay-Nedecký} and Jiří {Kudr} and Lukáš {Nejdl} and Darina {Mašková} and René {Kizek} and Vojtěch {Adam}",
  title="G-Quadruplexes as Sensing Probes",
  journal="MOLECULES",
  year="2013",
  volume="18",
  number="12",
  pages="14760--14779",
  doi="10.3390/molecules181214760",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/18/12/14760"
}