Publication detail

Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration

CHUDOBOVA, D. ČÍHALOVÁ, K. GURÁŇ, R. DOSTÁLOVÁ, S. ŠMERKOVÁ, K. VESELÝ, R. GUMULEC, J. MASAŘÍK, M. HEGER, Z. ADAM, V. KIZEK, R.

Original Title

Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration

Type

journal article in Web of Science

Language

English

Original Abstract

Background: Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one of the most serious health complications during a medical treatment. Therefore, this study is focused on the isolation, characterization, and identification of microorganisms prevalent in superficial wounds of patients (n=50) presenting with bacterial infection. Methods: After successful cultivation, bacteria were processed and analyzed. Initially the identification of the strains was performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on comparison of protein profiles (2-30 kDa) with database. Subsequently, bacterial strains from infected wounds were identified by both matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and sequencing of 16S rRNA gene 108. Results: The most prevalent species was Staphylococcus aureus (70%), and out of those 11% turned out to be methicillin-resistant (mecA positive). Identified strains were compared with patients' diagnoses using the method of artificial neuronal network to assess the association between severity of infection and wound microbiome species composition. Artificial neuronal network was subsequently used to predict patients' prognosis (n=9) with 85% success. Conclusions: In all of 50 patients tested bacterial infections were identified. Based on the proposed artificial neuronal network we were able to predict the severity of the infection and length of the treatment.

Keywords

Bacterial strains; MALDI-TOF; Sequencing; Superficial wounds

Authors

CHUDOBOVA, D.; ČÍHALOVÁ, K.; GURÁŇ, R.; DOSTÁLOVÁ, S.; ŠMERKOVÁ, K.; VESELÝ, R.; GUMULEC, J.; MASAŘÍK, M.; HEGER, Z.; ADAM, V.; KIZEK, R.

Released

1. 12. 2015

Publisher

Elsevier

ISBN

1413-8670

Periodical

BRAZ J INFECT DIS

Year of study

19

Number

6

State

Federative Republic of Brazil

Pages from

604

Pages to

613

Pages count

10

URL

https://www.sciencedirect.com/science/article/pii/S1413867015001889

Full text in the Digital Library

http://hdl.handle.net/11012/201034

BibTex

@article{BUT145197,
  author="Dagmar {Hegerová} and Kristýna {Číhalová} and Roman {Guráň} and Simona {Dostálová} and Kristýna {Šmerková} and Radek {Veselý} and Jaromír {Gumulec} and Michal {Masařík} and Zbyněk {Heger} and Vojtěch {Adam} and René {Kizek}",
  title="Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration",
  journal="BRAZ J INFECT DIS",
  year="2015",
  volume="19",
  number="6",
  pages="604--613",
  doi="10.1016/j.bjid.2015.08.013",
  issn="1413-8670",
  url="https://www.sciencedirect.com/science/article/pii/S1413867015001889"
}