Detail publikace

Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration

CHUDOBOVA, D. ČÍHALOVÁ, K. GURÁŇ, R. DOSTÁLOVÁ, S. ŠMERKOVÁ, K. VESELÝ, R. GUMULEC, J. MASAŘÍK, M. HEGER, Z. ADAM, V. KIZEK, R.

Originální název

Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background: Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one of the most serious health complications during a medical treatment. Therefore, this study is focused on the isolation, characterization, and identification of microorganisms prevalent in superficial wounds of patients (n=50) presenting with bacterial infection. Methods: After successful cultivation, bacteria were processed and analyzed. Initially the identification of the strains was performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on comparison of protein profiles (2-30 kDa) with database. Subsequently, bacterial strains from infected wounds were identified by both matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and sequencing of 16S rRNA gene 108. Results: The most prevalent species was Staphylococcus aureus (70%), and out of those 11% turned out to be methicillin-resistant (mecA positive). Identified strains were compared with patients' diagnoses using the method of artificial neuronal network to assess the association between severity of infection and wound microbiome species composition. Artificial neuronal network was subsequently used to predict patients' prognosis (n=9) with 85% success. Conclusions: In all of 50 patients tested bacterial infections were identified. Based on the proposed artificial neuronal network we were able to predict the severity of the infection and length of the treatment.

Klíčová slova

Bacterial strains; MALDI-TOF; Sequencing; Superficial wounds

Autoři

CHUDOBOVA, D.; ČÍHALOVÁ, K.; GURÁŇ, R.; DOSTÁLOVÁ, S.; ŠMERKOVÁ, K.; VESELÝ, R.; GUMULEC, J.; MASAŘÍK, M.; HEGER, Z.; ADAM, V.; KIZEK, R.

Vydáno

1. 12. 2015

Nakladatel

Elsevier

ISSN

1413-8670

Periodikum

BRAZ J INFECT DIS

Ročník

19

Číslo

6

Stát

Brazilská federativní republika

Strany od

604

Strany do

613

Strany počet

10

URL

https://www.sciencedirect.com/science/article/pii/S1413867015001889

Plný text v Digitální knihovně

http://hdl.handle.net/11012/201034

BibTex

@article{BUT145197,
  author="Dagmar {Hegerová} and Kristýna {Číhalová} and Roman {Guráň} and Simona {Dostálová} and Kristýna {Šmerková} and Radek {Veselý} and Jaromír {Gumulec} and Michal {Masařík} and Zbyněk {Heger} and Vojtěch {Adam} and René {Kizek}",
  title="Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration",
  journal="BRAZ J INFECT DIS",
  year="2015",
  volume="19",
  number="6",
  pages="604--613",
  doi="10.1016/j.bjid.2015.08.013",
  issn="1413-8670",
  url="https://www.sciencedirect.com/science/article/pii/S1413867015001889"
}