Publication detail

Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift

VACULOVIČOVÁ, M. ADAM, V. KIZEK, R.

Original Title

Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift

Type

journal article in Web of Science

Language

English

Original Abstract

Electrophoretic mobility shift assay (EMSA) is a well-established technique to monitor interactions between biomolecules particularly DNA and proteins. Even though numerous variations of this method have been presented, challenges in the form of detection sensitivity and/or variations in the stability of the formed complex still remain. With advances in the area of nanomaterials improvements in EMSA have been also suggested. Recently, Zhang and Wang (Electrophoresis 2015, 36, 1011-1015) presented electrophoretic mobility shift method for determination of number of DNA molecules conjugated to quantum dots (QDs), which was further utilized for calculation of enzymatic activity, sequence specific DNA detection, and neutral molecule quantification.

Keywords

DNA; Electrophoretic mobility shift assay; Protein; Quantum dots

Authors

VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Released

1. 1. 2015

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

36

Number

9-10

State

Federal Republic of Germany

Pages from

1084

Pages to

1085

Pages count

2

BibTex

@article{BUT145841,
  author="Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift",
  journal="Electrophoresis",
  year="2015",
  volume="36",
  number="9-10",
  pages="1084--1085",
  doi="10.1002/elps.201500168",
  issn="0173-0835"
}