Detail publikace

Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift

VACULOVIČOVÁ, M. ADAM, V. KIZEK, R.

Originální název

Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Electrophoretic mobility shift assay (EMSA) is a well-established technique to monitor interactions between biomolecules particularly DNA and proteins. Even though numerous variations of this method have been presented, challenges in the form of detection sensitivity and/or variations in the stability of the formed complex still remain. With advances in the area of nanomaterials improvements in EMSA have been also suggested. Recently, Zhang and Wang (Electrophoresis 2015, 36, 1011-1015) presented electrophoretic mobility shift method for determination of number of DNA molecules conjugated to quantum dots (QDs), which was further utilized for calculation of enzymatic activity, sequence specific DNA detection, and neutral molecule quantification.

Klíčová slova

DNA; Electrophoretic mobility shift assay; Protein; Quantum dots

Autoři

VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Vydáno

1. 1. 2015

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

36

Číslo

9-10

Stát

Spolková republika Německo

Strany od

1084

Strany do

1085

Strany počet

2

BibTex

@article{BUT145841,
  author="Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift",
  journal="Electrophoresis",
  year="2015",
  volume="36",
  number="9-10",
  pages="1084--1085",
  doi="10.1002/elps.201500168",
  issn="0173-0835"
}