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KUČERA, D. PERNICOVÁ, I. KOVALČÍK, A. KOLLER, M. MÜLLEROVÁ, L. SEDLÁČEK, P. MRAVEC, F. NEBESAROVA, J. KALINA, M. MÁROVÁ, I. KRZYŽÁNEK, V. OBRUČA, S.
Original Title
Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila
Type
journal article in Web of Science
Language
English
Original Abstract
This work explores molecular, morphological as well as biotechnological features of the highly promising polyhydroxyalkanoates (PHA) producer Halomonas halophila. Unlike many other halophiles, this bacterium does not require expensive complex media components and it is capable to accumulate high intracellular poly(3-hydroxybutyrate) (PHB) fractions up to 82% of cell dry mass. Most remarkably, regulating the concentration of NaCl apart from PHB yields influences also the polymer’s molecular mass and polydispersity. The bacterium metabolizes various carbohydrates including sugars predominant in lignocelluloses and other inexpensive substrates. Therefore, the bacterium was employed for PHB production on hydrolysates of cheese whey, spent coffee grounds, sawdust and corn stover, which were hydrolyzed by HCl; required salinity of cultivation media was set up during neutralization by NaOH. The bacterium was capable to use all the tested hydrolysates as well as sugar beet molasses for PHB biosynthesis, indicating its potential for industrial PHB production.
Keywords
Halomonas halophila; Halophiles; Lignocellulose hydrolysates; Morphology of bacterial cells; Polyhydroxyalkanoates
Authors
KUČERA, D.; PERNICOVÁ, I.; KOVALČÍK, A.; KOLLER, M.; MÜLLEROVÁ, L.; SEDLÁČEK, P.; MRAVEC, F.; NEBESAROVA, J.; KALINA, M.; MÁROVÁ, I.; KRZYŽÁNEK, V.; OBRUČA, S.
Released
1. 5. 2018
Publisher
Elsevier
ISBN
0960-8524
Periodical
BIORESOURCE TECHNOLOGY
Year of study
256
Number
1
State
Kingdom of the Netherlands
Pages from
552
Pages to
556
Pages count
5
URL
https://www.sciencedirect.com/science/article/pii/S0960852418302554
BibTex
@article{BUT147942, author="KUČERA, D. and PERNICOVÁ, I. and KOVALČÍK, A. and KOLLER, M. and MÜLLEROVÁ, L. and SEDLÁČEK, P. and MRAVEC, F. and NEBESAROVA, J. and KALINA, M. and MÁROVÁ, I. and KRZYŽÁNEK, V. and OBRUČA, S.", title="Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila", journal="BIORESOURCE TECHNOLOGY", year="2018", volume="256", number="1", pages="552--556", doi="10.1016/j.biortech.2018.02.062", issn="0960-8524", url="https://www.sciencedirect.com/science/article/pii/S0960852418302554" }