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KUČERA, D. PERNICOVÁ, I. KOVALČÍK, A. KOLLER, M. MÜLLEROVÁ, L. SEDLÁČEK, P. MRAVEC, F. NEBESAROVA, J. KALINA, M. MÁROVÁ, I. KRZYŽÁNEK, V. OBRUČA, S.
Originální název
Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
This work explores molecular, morphological as well as biotechnological features of the highly promising polyhydroxyalkanoates (PHA) producer Halomonas halophila. Unlike many other halophiles, this bacterium does not require expensive complex media components and it is capable to accumulate high intracellular poly(3-hydroxybutyrate) (PHB) fractions up to 82% of cell dry mass. Most remarkably, regulating the concentration of NaCl apart from PHB yields influences also the polymer’s molecular mass and polydispersity. The bacterium metabolizes various carbohydrates including sugars predominant in lignocelluloses and other inexpensive substrates. Therefore, the bacterium was employed for PHB production on hydrolysates of cheese whey, spent coffee grounds, sawdust and corn stover, which were hydrolyzed by HCl; required salinity of cultivation media was set up during neutralization by NaOH. The bacterium was capable to use all the tested hydrolysates as well as sugar beet molasses for PHB biosynthesis, indicating its potential for industrial PHB production.
Klíčová slova
Halomonas halophila; Halophiles; Lignocellulose hydrolysates; Morphology of bacterial cells; Polyhydroxyalkanoates
Autoři
KUČERA, D.; PERNICOVÁ, I.; KOVALČÍK, A.; KOLLER, M.; MÜLLEROVÁ, L.; SEDLÁČEK, P.; MRAVEC, F.; NEBESAROVA, J.; KALINA, M.; MÁROVÁ, I.; KRZYŽÁNEK, V.; OBRUČA, S.
Vydáno
1. 5. 2018
Nakladatel
Elsevier
ISSN
0960-8524
Periodikum
BIORESOURCE TECHNOLOGY
Ročník
256
Číslo
1
Stát
Nizozemsko
Strany od
552
Strany do
556
Strany počet
5
URL
https://www.sciencedirect.com/science/article/pii/S0960852418302554
BibTex
@article{BUT147942, author="KUČERA, D. and PERNICOVÁ, I. and KOVALČÍK, A. and KOLLER, M. and MÜLLEROVÁ, L. and SEDLÁČEK, P. and MRAVEC, F. and NEBESAROVA, J. and KALINA, M. and MÁROVÁ, I. and KRZYŽÁNEK, V. and OBRUČA, S.", title="Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila", journal="BIORESOURCE TECHNOLOGY", year="2018", volume="256", number="1", pages="552--556", doi="10.1016/j.biortech.2018.02.062", issn="0960-8524", url="https://www.sciencedirect.com/science/article/pii/S0960852418302554" }