Přístupnostní navigace
E-application
Search Search Close
Publication detail
KONEČNÁ, J. ROMANOVSKÁ, D. TRACHTOVÁ, Š.
Original Title
Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles
Type
journal article in Web of Science
Language
English
Original Abstract
Isolation of deoxyribonucleic acid is an important step in the molecular diagnostics of microorganisms. A high quality of isolated deoxyribonucleic acid is necessary for deoxyribonucleic acid amplification by the polymerase chain reaction. The conventional deoxyribinucleic acid isolation using phenol-chloroform extraction and deoxyribonucleic acid precipitation in ethanol is time-consumingand requires the use of oxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The ain of this work was to use two different types of deoxyribonucleic acid in separation mixtures were measured using ultraviolet spectrophotometry.
Keywords
Magnetic particles, Solid-phase extraction, Polymerase chain reaction, Deoxyribonucleic acid isolation
Authors
KONEČNÁ, J.; ROMANOVSKÁ, D.; TRACHTOVÁ, Š.
Released
1. 5. 2019
Publisher
Chemical papers
Location
Institute of Chemistry, Slovak Academy of Sciences
ISBN
1336-9075
Periodical
Chemical Papers
Year of study
120
Number
13
State
Slovak Republic
Pages from
1
Pages to
9
Pages count
URL
https://link.springer.com/article/10.1007/s11696-018-00675-9
BibTex
@article{BUT151274, author="Jana {Konečná} and Denisa {Langová} and Štěpánka {Trachtová}", title="Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles", journal="Chemical Papers", year="2019", volume="120", number="13", pages="1--9", doi="10.1007/s11696-018-00675-9", issn="1336-9075", url="https://link.springer.com/article/10.1007/s11696-018-00675-9" }