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KONEČNÁ, J. ROMANOVSKÁ, D. TRACHTOVÁ, Š.
Originální název
Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Isolation of deoxyribonucleic acid is an important step in the molecular diagnostics of microorganisms. A high quality of isolated deoxyribonucleic acid is necessary for deoxyribonucleic acid amplification by the polymerase chain reaction. The conventional deoxyribinucleic acid isolation using phenol-chloroform extraction and deoxyribonucleic acid precipitation in ethanol is time-consumingand requires the use of oxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The ain of this work was to use two different types of deoxyribonucleic acid in separation mixtures were measured using ultraviolet spectrophotometry.
Klíčová slova
Magnetic particles, Solid-phase extraction, Polymerase chain reaction, Deoxyribonucleic acid isolation
Autoři
KONEČNÁ, J.; ROMANOVSKÁ, D.; TRACHTOVÁ, Š.
Vydáno
1. 5. 2019
Nakladatel
Chemical papers
Místo
Institute of Chemistry, Slovak Academy of Sciences
ISSN
1336-9075
Periodikum
Chemical Papers
Ročník
120
Číslo
13
Stát
Slovenská republika
Strany od
1
Strany do
9
Strany počet
URL
https://link.springer.com/article/10.1007/s11696-018-00675-9
BibTex
@article{BUT151274, author="Jana {Konečná} and Denisa {Langová} and Štěpánka {Trachtová}", title="Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles", journal="Chemical Papers", year="2019", volume="120", number="13", pages="1--9", doi="10.1007/s11696-018-00675-9", issn="1336-9075", url="https://link.springer.com/article/10.1007/s11696-018-00675-9" }