Publication detail

Versatile digital polymerase chain reaction chip design, fabrication, and image processing

Li, H., Zhanga, H., Xua Y., Tureckova, A., Zahradnik, P., Chang, H., Neuzil, P.

Original Title

Versatile digital polymerase chain reaction chip design, fabrication, and image processing

Type

journal article in Web of Science

Language

English

Original Abstract

We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 x 9) mm(2) in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 x 2785 mu m(2) that contain reaction wells with a gap of a few mu m separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells' positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.

Keywords

Digital PCR; Silicon chip; Fluorescence image analysis; DNA counting; Microfluidic chip; Array

Authors

Li, H., Zhanga, H., Xua Y., Tureckova, A., Zahradnik, P., Chang, H., Neuzil, P.

Released

2. 1. 2019

Publisher

Elsevier B.V.

ISBN

0925-4005

Periodical

Sensors and Actuators B: Chemical

Year of study

283

Number

1

State

Swiss Confederation

Pages from

677

Pages to

684

Pages count

8

URL

https://doi.org/10.1016/j.snb.2018.12.072

BibTex

@article{BUT155223,
  author="Pavel {Neužil}",
  title="Versatile digital polymerase chain reaction chip design, fabrication, and image processing",
  journal="Sensors and Actuators B: Chemical",
  year="2019",
  volume="283",
  number="1",
  pages="677--684",
  doi="10.1016/j.snb.2018.12.072",
  issn="0925-4005",
  url="https://doi.org/10.1016/j.snb.2018.12.072"
}