Detail publikace

Versatile digital polymerase chain reaction chip design, fabrication, and image processing

Li, H., Zhanga, H., Xua Y., Tureckova, A., Zahradnik, P., Chang, H., Neuzil, P.

Originální název

Versatile digital polymerase chain reaction chip design, fabrication, and image processing

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 x 9) mm(2) in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 x 2785 mu m(2) that contain reaction wells with a gap of a few mu m separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells' positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.

Klíčová slova

Digital PCR; Silicon chip; Fluorescence image analysis; DNA counting; Microfluidic chip; Array

Autoři

Li, H., Zhanga, H., Xua Y., Tureckova, A., Zahradnik, P., Chang, H., Neuzil, P.

Vydáno

2. 1. 2019

Nakladatel

Elsevier B.V.

ISSN

0925-4005

Periodikum

Sensors and Actuators B: Chemical

Ročník

283

Číslo

1

Stát

Švýcarská konfederace

Strany od

677

Strany do

684

Strany počet

8

URL

https://doi.org/10.1016/j.snb.2018.12.072

BibTex

@article{BUT155223,
  author="Pavel {Neužil}",
  title="Versatile digital polymerase chain reaction chip design, fabrication, and image processing",
  journal="Sensors and Actuators B: Chemical",
  year="2019",
  volume="283",
  number="1",
  pages="677--684",
  doi="10.1016/j.snb.2018.12.072",
  issn="0925-4005",
  url="https://doi.org/10.1016/j.snb.2018.12.072"
}