Publication detail

Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation

BABRNÁKOVÁ, J. PAVLIŇÁKOVÁ, V. BRTNÍKOVÁ, J. SEDLÁČEK, P. PROSECKÁ, E. RAMPICHOVÁ, M. FILOVA, E. HEARNDEN, V. VOJTOVÁ, L.

Original Title

Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation

Type

journal article in Web of Science

Language

English

Original Abstract

Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties. Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented. Lipophilic BPL formed microspheres in porous scaffolds while reduced by half their swelling ratio. The resistance of collagen sponges to hydrolytic degradation in water depended strongly on chemical crosslinking varying from 60 min to more than one year. According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell–matrix interactions, and biocompatibility of the material. The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay. Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold. We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.

Keywords

Collagen Oxidized cellulose Chitosan Bovine platelet lysate Angiogenesis Fibroblast proliferation

Authors

BABRNÁKOVÁ, J.; PAVLIŇÁKOVÁ, V.; BRTNÍKOVÁ, J.; SEDLÁČEK, P.; PROSECKÁ, E.; RAMPICHOVÁ, M.; FILOVA, E.; HEARNDEN, V.; VOJTOVÁ, L.

Released

1. 7. 2019

Publisher

Elsevier

Location

Amsterdam, Netherlands

ISBN

0928-4931

Periodical

Materials Science and Engineering C-Materials for Biological Applications

Year of study

100

Number

1

State

Kingdom of the Netherlands

Pages from

236

Pages to

246

Pages count

11

URL

https://reader.elsevier.com/reader/sd/pii/S0928493118313948?token=D6A93EF019B232C90883451017697BFF030DDFCBE6A103EE582D53A10FD1B71E8F99C6E6FA0BDD0130F6BC8A951244BE

BibTex

@article{BUT155949,
  author="Johana {Muchová} and Veronika {Pavliňáková} and Jana {Brtníková} and Petr {Sedláček} and Eva {Prosecká} and Michaela {Rampichová} and Eva {Filova} and Vanessa {Hearnden} and Lucy {Vojtová}",
  title="Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation",
  journal="Materials Science and Engineering C-Materials for Biological Applications",
  year="2019",
  volume="100",
  number="1",
  pages="236--246",
  doi="10.1016/j.msec.2019.02.092",
  issn="0928-4931",
  url="https://reader.elsevier.com/reader/sd/pii/S0928493118313948?token=D6A93EF019B232C90883451017697BFF030DDFCBE6A103EE582D53A10FD1B71E8F99C6E6FA0BDD0130F6BC8A951244BE"
}