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BABRNÁKOVÁ, J. PAVLIŇÁKOVÁ, V. BRTNÍKOVÁ, J. SEDLÁČEK, P. PROSECKÁ, E. RAMPICHOVÁ, M. FILOVA, E. HEARNDEN, V. VOJTOVÁ, L.
Originální název
Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Crosslinked 3D porous collagen-polysaccharide scaffolds, prepared by freeze-drying, were modified with bovine platelet lysate (BPL) and evaluated in terms of chemical, physical and biological properties. Natural antibacterial polysaccharides like chitosan, chitin/chitosan-glucan complex and calcium salt of oxidized cellulose (CaOC) incorporated in collagen scaffolds affected not only chemo-physical properties of the composite scaffolds but also improved their biological properties, especially when BPL was presented. Lipophilic BPL formed microspheres in porous scaffolds while reduced by half their swelling ratio. The resistance of collagen sponges to hydrolytic degradation in water depended strongly on chemical crosslinking varying from 60 min to more than one year. According to in-vitro tests, chemically crosslinked scaffolds exhibited a good cellular response, cell–matrix interactions, and biocompatibility of the material. The combination of collagen with natural polysaccharides confirmed a significant positive synergistic effect on cultivation of cells as determined by MTS assay and PicoGreen method, as well as on angiogenesis evaluated by ex ovo Chick Chorioallantoic Membrane (CAM) assay. Contrary, modification only by BLP of pure collagen scaffolds exhibited decreased biocompatibility in comparison to unmodified pure collagen scaffold. We propose that the newly developed crosslinked collagen sponges involving bioactive additives could be used as scaffold for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
Klíčová slova
Collagen Oxidized cellulose Chitosan Bovine platelet lysate Angiogenesis Fibroblast proliferation
Autoři
BABRNÁKOVÁ, J.; PAVLIŇÁKOVÁ, V.; BRTNÍKOVÁ, J.; SEDLÁČEK, P.; PROSECKÁ, E.; RAMPICHOVÁ, M.; FILOVA, E.; HEARNDEN, V.; VOJTOVÁ, L.
Vydáno
1. 7. 2019
Nakladatel
Elsevier
Místo
Amsterdam, Netherlands
ISSN
0928-4931
Periodikum
Materials Science and Engineering C-Materials for Biological Applications
Ročník
100
Číslo
1
Stát
Nizozemsko
Strany od
236
Strany do
246
Strany počet
11
URL
https://reader.elsevier.com/reader/sd/pii/S0928493118313948?token=D6A93EF019B232C90883451017697BFF030DDFCBE6A103EE582D53A10FD1B71E8F99C6E6FA0BDD0130F6BC8A951244BE
BibTex
@article{BUT155949, author="Johana {Muchová} and Veronika {Pavliňáková} and Jana {Brtníková} and Petr {Sedláček} and Eva {Prosecká} and Michaela {Rampichová} and Eva {Filova} and Vanessa {Hearnden} and Lucy {Vojtová}", title="Synergistic effect of bovine platelet lysate and various polysaccharides on the biological properties of collagen-based scaffolds for tissue engineering: Scaffold preparation, chemo-physical characterization, in vitro and ex ovo evaluation", journal="Materials Science and Engineering C-Materials for Biological Applications", year="2019", volume="100", number="1", pages="236--246", doi="10.1016/j.msec.2019.02.092", issn="0928-4931", url="https://reader.elsevier.com/reader/sd/pii/S0928493118313948?token=D6A93EF019B232C90883451017697BFF030DDFCBE6A103EE582D53A10FD1B71E8F99C6E6FA0BDD0130F6BC8A951244BE" }