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BOUCHAL, P. ŠTRBKOVÁ, L. DOSTÁL, Z. CHMELÍK, R. BOUCHAL, Z.
Original Title
Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples
Type
journal article in Web of Science
Language
English
Original Abstract
We present geometric-phase microscopy allowing a multipurpose quantitative phase imaging in which the ground-truth phase is restored by quantifying the phase retardance. The method uses broadband spatially incoherent light that is polarization sensitively controlled through the geometric (Pancharatnam-Berry) phase. The assessed retardance possibly originates either in dynamic or geometric phase and measurements are customized for quantitative mapping of isotropic and birefringent samples or multi-functional geometric-phase elements. The phase restoration is based on the self-interference of polarization distinguished waves carrying sample information and providing pure reference phase, while passing through an inherently stable common-path setup. The experimental configuration allows an instantaneous (single-shot) phase restoration with guaranteed subnanometer precision and excellent ground-truth accuracy (well below 5 nm). The optical performance is demonstrated in advanced yet routinely feasible noninvasive biophotonic imaging executed in the automated manner and predestined for supervised machine learning. The experiments demonstrate measurement of cell dry mass density, cell classification based on the morphological parameters and visualization of dynamic dry mass changes. The multipurpose use of the method was demonstrated by restoring variations in the dynamic phase originating from the electrically induced birefringence of liquid crystals and by mapping the geometric phase of a space-variant polarization directed lens.
Keywords
Holographic microscopy, Geometric phase, Quantitative phase imaging,
Authors
BOUCHAL, P.; ŠTRBKOVÁ, L.; DOSTÁL, Z.; CHMELÍK, R.; BOUCHAL, Z.
Released
5. 3. 2019
Publisher
Springer Nature
ISBN
2045-2322
Periodical
Scientific Reports
Year of study
9
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
Pages to
11
Pages count
URL
http://link.springer.com/article/10.1038/s41598-019-40441-9
Full text in the Digital Library
http://hdl.handle.net/11012/179552
BibTex
@article{BUT156119, author="Petr {Bouchal} and Lenka {Štrbková} and Zbyněk {Dostál} and Radim {Chmelík} and Zdeněk {Bouchal}", title="Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples", journal="Scientific Reports", year="2019", volume="9", number="1", pages="1--11", doi="10.1038/s41598-019-40441-9", issn="2045-2322", url="http://link.springer.com/article/10.1038/s41598-019-40441-9" }