Detail publikace

Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples

BOUCHAL, P. ŠTRBKOVÁ, L. DOSTÁL, Z. CHMELÍK, R. BOUCHAL, Z.

Originální název

Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

We present geometric-phase microscopy allowing a multipurpose quantitative phase imaging in which the ground-truth phase is restored by quantifying the phase retardance. The method uses broadband spatially incoherent light that is polarization sensitively controlled through the geometric (Pancharatnam-Berry) phase. The assessed retardance possibly originates either in dynamic or geometric phase and measurements are customized for quantitative mapping of isotropic and birefringent samples or multi-functional geometric-phase elements. The phase restoration is based on the self-interference of polarization distinguished waves carrying sample information and providing pure reference phase, while passing through an inherently stable common-path setup. The experimental configuration allows an instantaneous (single-shot) phase restoration with guaranteed subnanometer precision and excellent ground-truth accuracy (well below 5 nm). The optical performance is demonstrated in advanced yet routinely feasible noninvasive biophotonic imaging executed in the automated manner and predestined for supervised machine learning. The experiments demonstrate measurement of cell dry mass density, cell classification based on the morphological parameters and visualization of dynamic dry mass changes. The multipurpose use of the method was demonstrated by restoring variations in the dynamic phase originating from the electrically induced birefringence of liquid crystals and by mapping the geometric phase of a space-variant polarization directed lens.

Klíčová slova

Holographic microscopy, Geometric phase, Quantitative phase imaging,

Autoři

BOUCHAL, P.; ŠTRBKOVÁ, L.; DOSTÁL, Z.; CHMELÍK, R.; BOUCHAL, Z.

Vydáno

5. 3. 2019

Nakladatel

Springer Nature

ISSN

2045-2322

Periodikum

Scientific Reports

Ročník

9

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

11

Strany počet

11

URL

http://link.springer.com/article/10.1038/s41598-019-40441-9

Plný text v Digitální knihovně

http://hdl.handle.net/11012/179552

BibTex

@article{BUT156119,
  author="Petr {Bouchal} and Lenka {Štrbková} and Zbyněk {Dostál} and Radim {Chmelík} and Zdeněk {Bouchal}",
  title="Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples",
  journal="Scientific Reports",
  year="2019",
  volume="9",
  number="1",
  pages="1--11",
  doi="10.1038/s41598-019-40441-9",
  issn="2045-2322",
  url="http://link.springer.com/article/10.1038/s41598-019-40441-9"
}