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PUENTE-MUNOZ, V. PAREDES, J., M. Resa, S. Vilchez, J., D. Žitňan, M. Miguel, D. Giron, M., D. Cuerva, J., M. Salto, R. Crovetto, L.
Original Title
New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures
Type
journal article in Web of Science
Language
English
Original Abstract
A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS fluorescence were measured. In these cells, GGDNBS fluorescence was correlated with the biothiol levels measured by an enzymatic method. Therefore, GGDNBS allows us to monitor changes in the levels of biothiols associated with ROS generation via single-cell bioimaging.
Keywords
molecular biology, chemistry
Authors
PUENTE-MUNOZ, V.; PAREDES, J., M.; Resa, S.; Vilchez, J., D.; Žitňan, M.; Miguel, D.; Giron, M., D.; Cuerva, J., M.; Salto, R.; Crovetto, L.
Released
7. 2. 2019
Publisher
NATURE PUBLISHING GROUP
Location
LONDON
ISBN
2045-2322
Periodical
Scientific Reports
Year of study
9
Number
2019
State
United Kingdom of Great Britain and Northern Ireland
Pages from
1659
Pages to
1669
Pages count
10
URL
https://www.nature.com/articles/s41598-018-38132-y
BibTex
@article{BUT161966, author="PUENTE-MUNOZ, V. and PAREDES, J., M. and Resa, S. and Vilchez, J., D. and Žitňan, M. and Miguel, D. and Giron, M., D. and Cuerva, J., M. and Salto, R. and Crovetto, L.", title="New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures", journal="Scientific Reports", year="2019", volume="9", number="2019", pages="1659--1669", doi="10.1038/s41598-018-38132-y", issn="2045-2322", url="https://www.nature.com/articles/s41598-018-38132-y" }