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PUENTE-MUNOZ, V. PAREDES, J., M. Resa, S. Vilchez, J., D. Žitňan, M. Miguel, D. Giron, M., D. Cuerva, J., M. Salto, R. Crovetto, L.
Originální název
New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS fluorescence were measured. In these cells, GGDNBS fluorescence was correlated with the biothiol levels measured by an enzymatic method. Therefore, GGDNBS allows us to monitor changes in the levels of biothiols associated with ROS generation via single-cell bioimaging.
Klíčová slova
molecular biology, chemistry
Autoři
PUENTE-MUNOZ, V.; PAREDES, J., M.; Resa, S.; Vilchez, J., D.; Žitňan, M.; Miguel, D.; Giron, M., D.; Cuerva, J., M.; Salto, R.; Crovetto, L.
Vydáno
7. 2. 2019
Nakladatel
NATURE PUBLISHING GROUP
Místo
LONDON
ISSN
2045-2322
Periodikum
Scientific Reports
Ročník
9
Číslo
2019
Stát
Spojené království Velké Británie a Severního Irska
Strany od
1659
Strany do
1669
Strany počet
10
URL
https://www.nature.com/articles/s41598-018-38132-y
BibTex
@article{BUT161966, author="PUENTE-MUNOZ, V. and PAREDES, J., M. and Resa, S. and Vilchez, J., D. and Žitňan, M. and Miguel, D. and Giron, M., D. and Cuerva, J., M. and Salto, R. and Crovetto, L.", title="New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures", journal="Scientific Reports", year="2019", volume="9", number="2019", pages="1659--1669", doi="10.1038/s41598-018-38132-y", issn="2045-2322", url="https://www.nature.com/articles/s41598-018-38132-y" }