Publication detail

Miniature and fully portable gradient capillary liquid chromatograph

LAM, S. COATES, L. HEMIDA, M. GUPTA, V. HADDAD, P. MACKA, M. PAULL, B.

Original Title

Miniature and fully portable gradient capillary liquid chromatograph

Type

journal article in Web of Science

Language

English

Original Abstract

A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight similar to 2.7 kg, without battery similar to 2.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm x 300 mu m I.D., 5 mu m ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of <0.7% (n = 10) and <3.3% (n = 10), respectively, and <0.1% (n = 10) and <2.3% (n = 10) respectively, for gradient elution mode. Detection was based upon a 255 nm light-emitting diode (LED) using one of two commercial capillary flow-cell options, namely a high sensitivity 12 nL Agilent capillary z-cell (HSDC) and a 45 nL Thermo Fisher Scientific UZ-ViewTM flow cell (UZFC). The HSDC, housed within a 3D printed detector arrangement, gave an effective pathlength of 1.01 mm (84% of nominal pathlength) and stray light of only 0.2%. Limits of detection for four test small molecule pharmaceuticals ranged from 65 to 101 mu g L-1 based upon a 316 nL injection volume, with separation efficiencies of between 18,000 and 29,700 N m(-1), with sub-4 min run times. The portable capillary LC system was successfully coupled to a small footprint portable mass spectrometer (Microsaic 4500 MiD) to demonstrate compatibility and 'point-of-need' miniaturised LC-MS capability.

Keywords

Miniaturisation; Portable; Capillary liquid chromatography; Ultraviolet light-emitting diode-based detector; Column heater; Portable mass spectrometer

Authors

LAM, S.; COATES, L.; HEMIDA, M.; GUPTA, V.; HADDAD, P.; MACKA, M.; PAULL, B.

Released

8. 3. 2020

ISBN

0003-2670

Periodical

ANALYTICA CHIMICA ACTA

Year of study

1101

Number

1

State

Kingdom of the Netherlands

Pages from

199

Pages to

210

Pages count

12

URL

https://doi.org/10.1016/j.aca.2019.12.014

BibTex

@article{BUT162605,
  author="Shing Chung {Lam} and Lewellwyn {Coates} and Mohamed {Hemida} and Vipul {Gupta} and Paul {Haddad} and Miroslav {Macka} and Brett {Paull}",
  title="Miniature and fully portable gradient capillary liquid chromatograph",
  journal="ANALYTICA CHIMICA ACTA",
  year="2020",
  volume="1101",
  number="1",
  pages="199--210",
  doi="10.1016/j.aca.2019.12.014",
  issn="0003-2670",
  url="https://doi.org/10.1016/j.aca.2019.12.014"
}