Detail publikace

Miniature and fully portable gradient capillary liquid chromatograph

LAM, S. COATES, L. HEMIDA, M. GUPTA, V. HADDAD, P. MACKA, M. PAULL, B.

Originální název

Miniature and fully portable gradient capillary liquid chromatograph

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight similar to 2.7 kg, without battery similar to 2.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm x 300 mu m I.D., 5 mu m ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of <0.7% (n = 10) and <3.3% (n = 10), respectively, and <0.1% (n = 10) and <2.3% (n = 10) respectively, for gradient elution mode. Detection was based upon a 255 nm light-emitting diode (LED) using one of two commercial capillary flow-cell options, namely a high sensitivity 12 nL Agilent capillary z-cell (HSDC) and a 45 nL Thermo Fisher Scientific UZ-ViewTM flow cell (UZFC). The HSDC, housed within a 3D printed detector arrangement, gave an effective pathlength of 1.01 mm (84% of nominal pathlength) and stray light of only 0.2%. Limits of detection for four test small molecule pharmaceuticals ranged from 65 to 101 mu g L-1 based upon a 316 nL injection volume, with separation efficiencies of between 18,000 and 29,700 N m(-1), with sub-4 min run times. The portable capillary LC system was successfully coupled to a small footprint portable mass spectrometer (Microsaic 4500 MiD) to demonstrate compatibility and 'point-of-need' miniaturised LC-MS capability.

Klíčová slova

Miniaturisation; Portable; Capillary liquid chromatography; Ultraviolet light-emitting diode-based detector; Column heater; Portable mass spectrometer

Autoři

LAM, S.; COATES, L.; HEMIDA, M.; GUPTA, V.; HADDAD, P.; MACKA, M.; PAULL, B.

Vydáno

8. 3. 2020

ISSN

0003-2670

Periodikum

ANALYTICA CHIMICA ACTA

Ročník

1101

Číslo

1

Stát

Nizozemsko

Strany od

199

Strany do

210

Strany počet

12

URL

https://doi.org/10.1016/j.aca.2019.12.014

BibTex

@article{BUT162605,
  author="Shing Chung {Lam} and Lewellwyn {Coates} and Mohamed {Hemida} and Vipul {Gupta} and Paul {Haddad} and Miroslav {Macka} and Brett {Paull}",
  title="Miniature and fully portable gradient capillary liquid chromatograph",
  journal="ANALYTICA CHIMICA ACTA",
  year="2020",
  volume="1101",
  number="1",
  pages="199--210",
  doi="10.1016/j.aca.2019.12.014",
  issn="0003-2670",
  url="https://doi.org/10.1016/j.aca.2019.12.014"
}