Publication detail

In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.

BREZNA, B. ŠMÍD, J. COSTA, J. RADVANCZKY, J. MAFRA, I. KUCHTA, T.

Original Title

In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.

Type

journal article in Web of Science

Language

English

Original Abstract

Ten published DNA-based analytical methods aiming at detecting material of almond (Prunus dulcis) were in silica evaluated for potential cross-reactivity with other stone fruits (Prunus spp.), including peach, apricot, plum, cherry, sour cherry and Sargent cherry. For most assays, the analysis of nucleotide databases suggested none or insufficient discrimination of at least some stone fruits. On the other hand, the assay targeting non-specific lipid transfer protein (Roder et al., 2011, Anal Chim Acta 685:74-83) was sufficiently discriminative, judging from nucleotide alignments. Empirical evaluation was performed for three of the published methods, one modification of a commercial kit (SureFood allergen almond) and one attempted novel method targeting thaumatin-like protein gene. Samples of leaves and kernels were used in the experiments. The empirical results were favourable for the method from Roder et al. (2011) and a modification of SureFood allergen almond kit, both showing cross-reactivity <10(-3) compared to the model almond

Keywords

Almond; DNA; Real-time PCR; Food; Allergen

Authors

BREZNA, B.; ŠMÍD, J.; COSTA, J.; RADVANCZKY, J.; MAFRA, I.; KUCHTA, T.

Released

1. 4. 2015

Publisher

Elsevier

ISBN

0890-8508

Periodical

MOLECULAR AND CELLULAR PROBES

Year of study

29

Number

2

State

United Kingdom of Great Britain and Northern Ireland

Pages from

99

Pages to

115

Pages count

16

URL

https://www.sciencedirect.com/science/article/pii/S0890850814000644

BibTex

@article{BUT163482,
  author="BREZNA, B. and ŠMÍD, J. and COSTA, J. and RADVANCZKY, J. and MAFRA, I. and KUCHTA, T.",
  title="In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.",
  journal="MOLECULAR AND CELLULAR PROBES",
  year="2015",
  volume="29",
  number="2",
  pages="99--115",
  doi="10.1016/j.mcp.2014.11.006",
  issn="0890-8508",
  url="https://www.sciencedirect.com/science/article/pii/S0890850814000644"
}